《植物生理学报》 2014, 50(12): 1863-1870
通信作者:张岁岐;E-mail: sqzhang@ms.iswc.ac.cn;Tel: 029-87010897
摘 要:
在水培条件下, 以水稻OsTIP1;1转基因油菜、空载体pHB转基因油菜以及野生型‘沪油15号’油菜为实验材料, 用细胞压力探针技术研究正常水分条件和PEG-6000模拟–0.2 MPa水分胁迫条件下, 液泡膜内在蛋白基因OsTIP1;1异位表达对油菜根皮层细胞水分关系参数的影响。结果表明, 与正常水分条件相比, 水分胁迫显著降低了细胞膨压(P)以及细胞水导(Lp), 而使细胞壁体积弹性模量(ε)和水分跨膜转运半时间(TW1/2)增加。在两种水分条件下, 细胞膨压(P)和细胞水导(Lp)都表现为OsTIP1;1转基因油菜>空载体pHB转基因油菜以及野生型油菜, 而OsTIP1;1转基因油菜的TW1/2显著小于其他两种油菜, ε值在正常水分条件下明显小于其他两种油菜, 水分胁迫后不同材料间差异不显著。说明水稻液泡膜内在蛋白基因OsTIP1;1在转基因油菜中异位表达可提高油菜根系的水分运输效率。关键词:油菜; 液泡膜内在蛋白; 细胞压力探针; 水分胁迫; 水力学参数
收稿:2014-10-15 修定:2014-11-17
资助:国家高技术研究(863)发展计划项目(2011AA100504)和国家自然科学基金面上项目(31270873)。
Corresponding author: ZHANG Sui-Qi; E-mail: sqzhang@ms.iswc.ac.cn; Tel: 029-87010897
Abstract:
Plant materials including wild-type (WT) Brassica napus cv. ‘Huyou 15’, transgenic B. napus expressing OsTIP1;1 or pHB empty vector were cultivated by using hydroponic culture system. The cell pressure probe was employed to investigate the effects of tonoplast intrinsic protein gene (OsTIP1;1) on water related parameters of primary root cortex cells among three B. napus materials under normal and water stress conditions. The water stress was simulated with PEG-6000 with Ψs –0.2 MPa. The results indicated that water stress decreased the root cortex cell turgor and the cell hydraulic conductivity, but increased the volumetric elastic modulus of cell wall and the half time of water exchange of three B. napus materials by contrast to normal conditions. Under different water conditions, the cell turgor and the cell hydraulic conductivity of OsTIP1;1 transgenic B. napus were greater than those of pHB transgenic B. napus and WT. And the half time of water exchange of OsTIP1;1 transgenic B. napus was lower than that of pHB transgenic B. napus and WT. The volumetric elastic modulus of OsTIP1;1 transgenic B. napus are lower than other two materials under normal condition, and the differences of elastic modulus among the three materials were not significant under water stress condition. The results suggested that ectopic expression of OsTIP1;1 in B. napus can improve water transport efficiency of the transgenic plants.Key words: oilseed rape; tonoplast intrinsic protein; cell pressure probe; water stress; hydraulic properties
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